表达Ⅰ型耐热肠毒素的重组大肠杆菌对7日龄仔猪肠道免疫、屏障及抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2017.09.023

Abstract

Abstract:

This experiment was conducted to investigate the effects of recombinant escherichia coli expressing heat-stable enterotoxin (STa) on intestinal immune and barrier function and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group, STa group (2×10⁹ CFU E. coli LMG194-STa), LMG194 group (2×10⁹ CFU E. coli LMG194), and K88 group (2×10⁹ CFU E. coli K88). The pigs were fed with artificial milk and slaughtered 7 days later. The result showed that, compared with the control group, STa group significantly reduced IFN-γ and IL-4 gene transcription levels (P<0.05), and increased VNN1 transcription level in ileum (P<0.05). STa group also significantly reduced gene transcription levels of Villin and MMP3 in ileum (P<0.05) and protein expression levels of Occludin and Claudin-1 in jejunum (P<0.05). STa group significantly reduced activity of glutathione peroxidase (GSH-Px) in duodenum and ileum (P<0.05), and significantly reduced activity of myeloperoxidase (MPO) in ileum (P<0.05), and had a higher production of malondialdehyde (MDA) in duodenum (P<0.05). These results suggest that recombinant Escherichia coli expressing STa can reduce intestinal immune and barrier function and antioxidant capacity of 7 days old piglets.

CLC Number:

S855.1

Lü Yang, ZHANG Lin, LI Xue-ni, ZHAO Di, YI Dan, CHEN Hong-bo, WU Tao. Effects of Recombinant Escherichia coli Expressing Heat-stable Enterotoxin on Intestinal Immune and Barrier Function and Antioxidant Capacity of Seven Days Old Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1761-1768.

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Effects of Recombinant Escherichia coli Expressing Heat-stable Enterotoxin on Intestinal Immune and Barrier Function and Antioxidant Capacity of Seven Days Old Piglets

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